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; with DNA omitted) had been superimposed with regard for their NTase domains and after that offset laterally to expose the big motion in the OB domain induced by nick recognition.
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Ellenberger and co-staff (8) had intuited accurately this kind of movement of the OB area after they solved the crystal structure from the much bigger human DNA ligase I (HuLig1; 919 amino acids) bound to an adenylylated nick. Their landmark structure was the first to doc the circumferential envelopment with the DNA duplex by a ligase clamp along with the resulting bending and distortion of your duplex that pressure the base pairs on the three′-OH side on the nick into an RNA-similar to a helical conformation (8). Equivalent distortions were noticed inside the ChVLig·DNA elaborate (7).
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